Diabetes Research Center (DRC)
Washington University in St. Louis

Mass Spectrometry Core

The Mass Spectrometry (MS) Core provides MS analyses to DRC Investigators in order to quantify and/or to determine structures of diabetes-related biomolecules. The Core increases efficiency and cost effectiveness by providing centralized, standardized analyses to study molecular mechanisms of the pathogenesis of diabetes, its risk factors, and its complications. A major goal of the core is to promote use of MS methods in diabetes research by efforts in training, collaboration, development, service, and dissemination.

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John W. Turk, M.D., Ph.D.

John W. Turk, M.D., Ph.D.

Director
Professor of Medicine

Internal Medicine - Division of Endocrinology, Metabolism & Lipid Research

Mailing Address   Campus Box 8127
Office Phone:   (314) 362-8190
Fax:   (314) 362-7617
E-mail address:   jturk@wustl.edu

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Kevin Yarasheski, Ph.D.

Kevin Yarasheski, Ph.D.

Co-Director
Professor of Medicine

Internal Medicine - Division of Endocrinology, Metabolism & Lipid Research

Mailing Address   Campus Box 8127
Office Phone:   (314) 362-8173
Fax:   (314) 362-7617
E-mail address:   key@wustl.edu

 Please contact Dr. Yarasheski for consultation on isotope ratio mass spectrometry & clinical/translational studies.

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Contacts

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For Sample Submission:

Jan Crowley, B.S. 

Jan Crowley, B.S.

Head Technical Specialist

Internal Medicine - Division of Endocrinology, Metabolism & Lipid Research

Mailing Address   Campus Box 8127
Office Phone:   (314) 362-7878
Fax:   (314) 362-7617
E-mail address:   jcrowley@wustl.edu

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For Consultation on ESI/MS/MS Analysis of Complex Lipids:

Fong-Fu Hsu, Ph.D.

Fong-Fu Hsu, Ph.D.

Assistant Director
Research Professor of Medicine

Internal Medicine - Division of Endocrinology, Metabolism & Lipid Research

Mailing Address   Campus Box 8127
Office Phone:   (314) 362-7641
Fax:   (314) 362-7617
E-mail address:   fong@wustl.edu

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Services

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Complex Lipids: Qualitative and Quantitative Analyses

ESI/MS(/MSn) analyses for structural identification and quantification of triacylglycerols, glycerophospholipids, and sphingolipids (and their subclasses) in biological samples.

Triacylglycerol

References

LC/MS/MS, GC/MS and GC/MSn for qualitative and quantitative analysis of fatty acids, sterols, complex lipids, and their metabolites.  

GC/MS Single ion monitoring chromatogram of TBDMS derivatives of Short Chain Fatty Acids (SCFA)

References

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Lipid Bioinformatics

The Core makes available a set of computer algorithms designated LipidQA for automated identification and quantitation of glycerophospholipid molecular species obtained using a variety of tandem mass spectrometers. The Core provides the software and advice on its operation and use to interested investigators.

Flow chart of algorithm for processing MS data to identify and quantitate complex lipid species

References

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ESI/MS/MS and LC/MS/MS Quantitation of Lipid Mediators

Quantitation of ceramide, sphingomyelin, and lysophosphatidylcholine by constant neutral loss ESI/MS/MS scanning during ER stress-induced apoptosis of pancreatic islet ß-cells and vascular myocytes. .

References

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ESI/MS/MS and LC/MS/MS Quantitation of Lipid Metabolites

Qualitative and quantitative analysis of long chain acylcarnitines and glycerophospholipids in tissues from genetically modified mice (iPLA2ß or iPLA2γ null) subjected to diabetogenic diets.

References

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MALDI/TOF/MS and LC/ESI/MS(/MSn) of Proteins and Peptides

Identify proteins and their post-translational modifications from enzymatic digests and peptide mixtures. Collaborative application of Stable Isotope Labeling with Amino Acids in Cell Culture (SILAC) and Stable Isotope Labeling Tandem (SILT) mass spectrometry methods to quantify changes in targeted protein expression.

References

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Metabolic Studies of Disposition of Stable Isotope Labeled Substrates

GC/MS, GC/MSn analyses of chemically derivatized mixtures of amino acids, fatty acids, or glucose after stable isotope labeled precursor administration to animals and humans are used to determine 13C-, 15N-, 2H-enrichment in biological samples. Gas chromatography-combustion-reduction IRMS analyses of 13C-amino acids, fatty acids and glucose enrichment for quantifying rates of substrate turnover, incorporation and clearance. Gas-IRMS analyses of 13CO2 in breath is used to quantify substrate oxidation rate.

References

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Identification of Unknown Biologically Active Substances

Facility staff can assist investigators with designing mass spectrometry-based structure and molecular weight determination strategies and mass spectra interpretation for characterizing and identifying unique bioactive molecules extracted from biological matrices .

References

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Training and Education

Tutorials

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Midwest Mass Spectrometry Discussion Group

The Mass Spectrometry Discussion Group meets regularly during the academic year and provides a venue for DRC trainees, investigators and outside guests to present novel ideas, discuss principles and applications of mass spectrometry to diabetes-related problems. 

MWMS Website

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Chargeback

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The DRC Mass Spectrometry Core conducts many routine and custom-designed mass spectrometry-based analyses for targeted diabetes-related biomolecules and biomolecular profiling experiments.  PIs must discuss their analyte(s) of interest and experimental design with the Core Director (Turk) or Co-Director (Yarasheski) PRIOR to submitting samples for analysis. A list of routine analytes, sample submission instructions, and contact information are available here.

When composing budget requests for new grant applications, the PI should contact the DRC Core Director or Co-Director.  The new grant application budget request should include effort/salary support for MS technical staff, MS instrumentation maintenance, service, repair, parts, and consumable supplies that are based on the anticipated amount of technical support and analytical work required. The Directors will help the PI compose the budget request, budget item justification, and letters of support or collaboration. This flat-fee option may be the most economical and should be pursued first and well in advance (~4wks) of budget submission deadlines.

Chargeback rates (Table) listed reflect cost per hour of instrument time used or staff service time provided . Collaborator is defined as co-authorship for DRC Mass Spectrometry Core staff on any abstract, preliminary report, manuscript, patent application that incorporates analytical results generated using MS instrumentation or service provided.

Instrumentation-Service-Analysis Provided

charge

Gas Chromatography-Mass Spectrometry

 

MS Staff conducts the analysis-operates the instrument

$40/hr

PI/Technician conducts the analysis-operates the instrument

20

 

 

Gas Chromatography-Tandem Mass Spectrometry

 

MS Staff conducts the analysis-operates the instrument

60

PI/Technician conducts the analysis-operates the instrument

30

 

 

Matrix Assisted Laser Desorption Ionization Mass Spectrometry

 

MS Staff conducts the analysis-operates the instrument

30

PI/Technician conducts the analysis-operates the instrument

15

 

 

Gas Isotope Ratio Mass Spectrometry

40

Direct Injection-Electrospray-Tandem Mass Spectrometry

30

Liquid Chromatography-Electrospray-Tandem Mass Spectrometry

40

 

 

Sample Preparation: support, advice, mass spectrum interpretation

60

Training (instrument operation, sample prep, data analysis, database searching)

50

Provision of specialized MS-related services (repairs, maintenance)

50

General lab space-minor equipment use

30

 

 

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Sample Submission Information

NIH / NCRR Mass Spectrometry Resource