Diabetes Research Center (DRC)
Washington University in St. Louis

Immunology of Type 1 Diabetes Core

The role of the Immunology of Type 1 Diabetes Core is to provide logistic support to investigators examining autoimmune or type 1 diabetes mellitus (T1DM) or other endocrine autoimmunities. The main purpose is to foster fundamental studies on T1DM through support for acquisition and use of diabetogenic strains of mice, islet isolation, and distribution of cells and antibodies relevant for immunological studies of T1DM. .

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Emil R. Unanue, M.D.

Emil R. Unanue, M.D.

Director
Paul & Ellen Lacy Professor of Pathology & Immunology

Department of Pathology and Immunology

Mailing Address   Campus Box 8118
660 S. Euclid Ave.
St. Louis, MO 63110
Office Phone:   (314) 362-7440
E-mail address:   unanue@wustl.edu

Dr. Unanue's Faculty Page (Pathology & Immunology)

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Contacts

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Inbred Mouse Component

Katherine Frederick

Staff Scientist
Laboratory Supervisor

Department of Pathology and Immunology

Mailing Address   Campus Box 8118
660 S. Euclid Ave.
St. Louis, MO 63110
Office Phone:   (314) 362-7412
E-mail address:   kfred@wustl.edu

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Genetics Component

Michael White

Staff Scientist
Laboratory Supervisor - Transgenic Facility

Department of Pathology and Immunology

Mailing Address   Campus Box 8118
660 S. Euclid Ave.
St. Louis, MO 63110
Office Phone:   (314) 362-8746
E-mail address:   white@wustl.edu

 

Robert Schreiber, M.D.

Alumni Professor of Pathology & Immunology

Department of Pathology and Immunology

Mailing Address   Campus Box 8118
660 S. Euclid Ave.
St. Louis, MO 63110
Office Phone:   (314) 362-8747
E-mail address:   schreiber@wustl.edu

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Cellular Component

Boris Calderon, M.D.

Instructor in Pathology and Immunology

Department of Pathology and Immunology

Mailing Address   Campus Box 8118
660 S. Euclid Ave.
St. Louis, MO 63110
Office Phone:   (314) 362-7526
E-mail address:   calderon@wustl.edu

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Services

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Maintenance/provision of various inbred mouse strains:

The Core maintains in the enhanced barrier facility and provides to investigators a number of inbred lines relevant for diabetes and autoimmune research:

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Training in and maintenance and testing of diabetogenic strains:

Investigators and their technical staff are instructed on breeding strategies for maintenance of NOD mice and other inbred strains of relevance to T1DM. In addition, investigators and their technical staff are instructed on applications of routine assays for beta cell function, including glucose assays, glucose tolerance tests in these mice.

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Training for the isolation of islets of Langerhans:

The Core provides assistance and training in the isolation of islets. We use a modified rapid islet isolation method developed by Salvalaggio et al. (2002) from the original protocol developed by Paul Lacy. More than 95% overall purity is readily obtainable using this protocol.

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Generation of new diabetogenic mouse strains:

The Immunology of T1DM Core leverages an existing highly successful institutional core in the Department of Pathology and Immunology for cost-effective generation of new transgenic and knockout/knockin lines specifically in the Balb/c and NOD strains using ES lines derived from these lines. Transgenic mice are generated by conventional injection of conventional transgenic construct DNA, lentiviral constructs, or bacterial artificial chromosomes (BACs) into fertilized eggs. Gene-targeted mice are generated via conventional microinjection of ES cells into blastocysts including blastocyst complementation. Other services include re-derivation of mouse embryos and cryopreservation of 2-cell embryos.

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Provision of isolated cells and cell lines relevant for immunological investigations of T1DM:

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Provision of monoclonal antibodies relevant for immunological investigations of T1DM (aliquots of the cells are provided):

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Chargeback

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Service Charges
Cell Lines $25
Monoclonal Antibodies $100
Mouse Genetics Services  
     Injections  $1,149
     Rederivation  $128
     Cryopreservation  $189