|
|
|
Clay Semenkovich, M.D.
314-362-7617
314-362-7641
(fax)
csemenko@wustl.edu
Core Lab Contacts:
Trey Coleman
314-747-8282
tcoleman@wustl.edu
Lab Location:
842 Southwest Tower
|
Mouse Phenotyping
With the increasing use of transgenic and ES cell technology to produce models of human physiology
and human diseases in mice, the ability to use sophisticated and technically demanding experimental
methods to phenotype mouse models in vivo has become a priority for diabetes investigators.
The Mouse Phenotyping Core provides members of the DRTC with cost-effective technical services to
characterize the metabolic phenotypes of genetically engineered mice relevant to diabetes.
Core Services
- Biochemical analyses relevant to diabetes:
- Blood glucose
- Serum glucose, cholesterol, triglycerides, and free fatty acids
- Serum lipoproteins. (Mouse VLDL, IDL/LDL and HDL)
- Glucose and insulin tolerance testing
- Noninvasive determination of body composition in living mice
- Lean tissue mass B
- Bone mineral density
- Fat mass
- Metabolic rate by indirect calorimetry
- Measure blood pressure noninvasively in living, unanesthetized mice
- Perform hyperinsulinemic euglycemic clamping in mice
- Determine ex vivo glucose transport in skeletal muscle
- Implant osmotic pumps for the infusion of leptin and other peptides
- Determine insulin secretion by in situ perfusion of mouse pancreas
- Perform quantitative atherosclerosis analyses of mouse aorta
- Train investigators in the breeding, maintenance, and analysis of mouse colonies relevant to diabetes research.
Training includes:
- Determining sex of pups, common techniques for identifying pups, and instructions for weaning (at 21 days of life)
- Instruction in rapid processing of large numbers tail samples for preparation of DNA
- PCR genotyping of animals
- Development of breeding strategies for generating mixed mutant mice, recognizing non-Mendelian inheritance, and practical instructions for identifying females unlikely to successfully raise pups
- Instruction in blood drawing in mice either via the tail vein or lateral saphenous vein
- Instruction in identifying current resources relevant to mouse physiology and phenotyping
Useful Resources:
|
